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Title of Journal: JAMA

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Abbravation: Journal of the American Medical Association

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American Medical Association

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0002-9955

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PRELIMINARY CLINICAL EVALUATION OF THROMBIN RECOVERY TEST

Authors: L. A. Sternberger,

Publish Date: 1952/12/20
Volume: 150, Issue:16, Pages: 1591-1593
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Abstract

The laboratory diagnosis of thrombosis has been difficult because of the absence in circulating blood of the two essential products of coagulation, thrombin and fibrin. The fibrin is fixed at a local site, while the excess of thrombin formed becomes inactivated immediately by antithrombic substances. Recently a method for recovering such inactivated thrombin has been developed,1 and elevated values have been demonstrated in thrombosis produced experimentally in rabbits. The method has now been applied to patients, and the present paper reports on these clinical observations.METHODS  The recovery of thrombin is accomplished by dissociating the thrombin-antithrombin complex with alkali slightly above freezing temperature and by preventing its reassociation upon neutralization through the addition of ethyl alcohol to a final concentration of 25%. Nine milliliters of venous blood are obtained with siliconized syringes2 containing 1.0 ml. of 4% sodium citrate solution. Siliconized needles (19 gauge) are used. The plasma

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References

citation_title=The Action of Heparin, Serum Albumin (Crystalline), and Salmine on Blood-Clotting Mechanisms (In Vitro); citation_author=Ferguson , J. H.;Am. J. Physiol.; citation_year=1940; citation_volume=130; citation_issue=Oct.; citation_pages=759-770 (;

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  7. THEORY OF SERUM THERAPY; CONTRIBUTION FROM THE BACTERIOLOGIC LABORATORY OF THE CITY OF PHILADELPHIA.
  8. THE ERYTHROCYTE SEDIMENTATION RATE: THE ADEQUACY OF A SIMPLE TEST AND ITS PRACTICAL APPLICATION IN CLINICAL MEDICINE
  9. Experimental Investigations in Serum Allergy with Reference to the Etiology of Rheumatic Joint Diseases
  10. Grayson's Diseases of the Nose
  11. Tubal Ligation
  12. THE HUMIDITY OF SCHOOLROOMS
  13. THE PROPER MATERIAL FOR MEDICAL SOCIETIES.
  14. BERLIN
  15. Cholera in Zimbabwe
  16. Access to Care in Regionalized Health Care Systems
  17. WHAT IS FEVER?
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  19. LETTER FROM PARIS.
  20. References and Reviews
  21. Preexposure Prophylaxis for HIV Infection
  22. THE STUDY OF NORMALS
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