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Detecting Plant Viruses in Their Vectors

Authors: Roger T. Plumb,

Publish Date: 1989
Volume: , Issue:, Pages: 191-208
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Until the advent of such techniques as electron microscopy and serology, the principal way of determining whether a suspected virus vector contained a plant-infecting virus was to allow it access to a plant host known to be susceptible to the virus. In other words, a bioassay of the vector for the appropriate virus was performed. An alternative approach was to use extracts of whole suspected vectors to inoculate susceptible test species mechanically. The latter method was obviously of limited use, and its usefulness was greatly affected by low virus concentrations in potential vectors and inactivation of virus in vector extracts.



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