RAPD typing of northeast Atlantic minke whale Ba

Authors: Martinez I Elvevoll E O Haug T

Publish Date: 1997/06/01

Volume: 54, Issue: 3, Pages: 478-484

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Abstract

This work describes the conditions for applying the arbitrary amplification of polymorphic DNA for stock determination in the minke whale Out of 192 primers screened only 36 were useful to detect genomic DNApolymorphisms Seven of these primers were used to type 31 minke whales caught in the waters around Spitsbergen n=7 Bear Island n=8 Finnmark n=8 and LofotenVesterålen n=8 Our results showed first a low percentage of primers 19 revealing polymorphisms secondly a high number of polymorphic bands with high frequencies 6 out of 14 had a frequency higher than 077 and 3 of those had a frequency higher than 090 and thirdly an average fraction of matching bands that was sometimes greater between than within areas None of these findings support the view that the 31 typed animals belonged to distinct breeding stocks thus emphasizing the importance of carrying out a broader study including more animals locations years and techniques of analysis in order to clarify the number and borderlines of breeding stocks in the northeast Atlantic

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