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Springer, Dordrecht
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Authors: S Felitti K Shields M Ramsperger P Tian T Webster B Ong T Sawbridge G Spangenberg
Publish Date: 2004
Volume: , Issue: , Pages: 145-153
Abstract
A genomic resource of 9411 expressed sequence tags ESTs representing 5038 genes has been established for Neotyphodium and Epichloë endophytes A cDNA spotted microarray interrogating 4195 Neotyphodium and 920 Epichloë genes EndoChip has been generated It provides a tool for highthroughput transcriptional profiling of endophytes genomespecific gene expression analysis and expression profiling of novel endophyte genes as well as transcriptome analysis in the grassendophyte association Microarraybased gene expression analysis using RNA from in vitro grown N coenophialum and N lolii endophytes demonstrated its utility in comparative transcriptome analysis of both endophyte species Additional proof of concept was obtained in using the Endo Chip to analyse genome widegene expression in endophytes grown in vitro and in planta and for gene expression profiling of novel genes based on coordinated expression with genes of known cellular functions Northern hybridisation analysis allowed for validation of microarraybased gene expression data In combination with established unigene microarrays for the host grasses it provides a tool for the dissection of the grassendophyte association at the transcriptome level
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