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Publisher
Springer, Dordrecht
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Authors: Antonie M Verschoor Harry Boonstra Thijs Meijer
Publish Date: 2005
Volume: , Issue: , Pages: 535-549
Abstract
We present a protocol and calculation methods for the determination of zooplankton ingestion and assimilation rates with stable isotope tracers These methods have been developed from experiments with the rotifer Brachionus calyciflorus that had been fed 13Clabelled Scenedesmus obliquus Stable isotope tracers offer the same advantages as radioisotopes These include the possibility for direct and accurate quantification of ingestion and assimilation rates short sample analysis times and low animal densities requirements However the use of stable isotope tracers requires relatively long sample preparation times and specialist equipment and is thus relatively costly for most laboratories The application of stable isotope tracers in zooplankton feeding studies offers several advantages in comparison with radioisotopes Firstly they do not emit harmful radiation and can therefore be applied safely both in the laboratory and in the field Secondly the samples can be dried for safe storage and easy transportation Thirdly no aggressive chemicals are required for sample analysis
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