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Title of Journal: Environ Biol Fish

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Abbravation: Environmental Biology of Fishes

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Springer Netherlands

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DOI

10.1007/s11467-013-0321-0

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1573-5133

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Assessing genetic diversity of wild populations of

Authors: Jun Song Zhaobin Song Bisong Yue Wenjing Zheng
Publish Date: 2006/07/05
Volume: 77, Issue: 1, Pages: 79-86
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Abstract

Prenantȁ9s schizothoracin Schizothorax prenanti an endemic fish to China has undergone a dramatic decline in numbers due to human impacts We studied its genetic diversity in three tributaries of the Yangtze River the Qingyi River which has many hydropower dams and the Dadu River and Muli River where many hydropower dams are being proposed Using amplified fragment length polymorphism AFLP 621 loci were amplified with seven AFLP primer combinations in 45 individuals The loci were highly polymorphic and heterozygous 87 polymorphism 30 heterozygosity The genetic distances within populations were large The analysis of molecular variance demonstrated that most variation occurred within populations The estimated fixation index Φst value averaged over all polymorphic loci across the three rivers was 00837 indicating a moderate genetic differentiation The differentiations between populations were significant and population structure was strong The results suggested that China had wild populations of Prenantȁ9s schizothoracin with considerable genetic diversity in the Muli Dadu and Qingyi rivers The proposals to dam these rivers should take into account the importance of conserving their genetic qualityThis work was funded by Science and Technology Bureau of Sichuan Province China No 03JY0290712 We would like to thank Taiming Yan Tianming Xie and Chunlin He for their help in sample collecting and Fangdong Zou and Zen Zhang for their help during the experiment We are grateful to Mart R Gross Aviva Patel and two anonyms for their helpful comments on the manuscript and Katherine Pieczora and Sean Clark for their help in English writing


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