Paper Search Console

Home Search Page Alphabetical List About Contact

Journal Title

Title of Journal: Eur J Clin Microbiol Infect Dis

Search In Journal Title:

Abbravation: European Journal of Clinical Microbiology & Infectious Diseases

Search In Journal Abbravation:

Publisher

Springer-Verlag

Search In Publisher:

DOI

10.1002/zaac.200900313

Search In DOI:

ISSN

1435-4373

Search In ISSN:
Search In Title Of Papers:

Detection and quantification of hepatitis B virus DNA by SYBR green real-time polymerase chain reaction

Authors: D. Olioso, M. Boaretti, M. Ligozzi, G. Lo Cascio, R. Fontana,

Publish Date: 2006/11/25
Volume: 26, Issue:1, Pages: 43-50
PDF Link

Abstract

A single-round real-time polymerase chain reaction (PCR) assay based on SYBR green dye technology for the detection and quantification of hepatitis B virus (HBV) DNA in serum was evaluated and compared with a qualitative nested PCR and the Cobas Amplicor HBV Monitor assay (Roche Molecular Diagnostics, Milan, Italy). The performance of the real-time PCR assay was evaluated in a routine clinical laboratory setting with a total of 212 clinical specimens. The sensitivity of the real-time PCR corresponded to 31 IU/ml (70 copies/ml), and comparison with the qualitative nested PCR showed significant concordance for 94% of samples. The linear curve over 7 log units, spanning 103–109 IU/ml (2.28 × 103 to 2.28 × 109 copies/ml), was observed in the quantitative determination. The interexperimental variability coefficient of the assay ranged from 0.22 to 0.39 and the intraexperimental variability coefficient from 0.24 to 0.41. By excluding values outside of the dynamic ranges of both tests, the HBV Monitor and the real-time PCR gave an agreement within ±1 log unit for 90% of samples, while those for the remaining 10% were found to be above 1 log unit but less than 1.5 log units. When the results inside and outside the dynamic range of the HBV Monitor were examined, 90% of the results were in agreement. In conclusion, the real-time PCR based on SYBR green technology proved suitable for routine diagnostic purposes, showing good sensitivity, high specificity, high reproducibility, and good linearity over a broad dynamic range of quantification.


Keywords:

References


.
Search In Abstract Of Papers:
Other Papers In This Journal:


Search Result:



Help video to use 'Paper Search Console'