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Publisher
Springer, Dordrecht
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Authors: Maya Kuchenbecker Bernd Rehberger Michael Schriek Judith Gackowski Ralf Fehrenbach
Publish Date: 2007
Volume: , Issue: , Pages: 57-61
Abstract
Due to regulatory requirements today’s stateoftheart cell culture production processes are performed in well defined serum or proteinfree media Nevertheless development of recombinant production cell lines is often performed under serumcontaining conditions Accordingly after clonal selection the cells have to be adapted to serumfree medium which bears the risk of losing productivity or product quality and significantly extends development time Through extensive medium screening and testing of multiple transfection methods a CHO dhfr host cell based procedure was established allowing the serumfree performance of all steps being essential for the development of a recombinant cell line
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