Authors: Zhen Yang Jiemei Wang Lichun Wang Long Chen Chang Tu Chufang Luo Anli Tang ShengMing Wang Jun Tao
Publish Date: 2007/01/13
Volume: 23, Issue: 2, Pages: 121-127
Abstract
Recent study suggested that endothelial progenitor cells EPCs a novel therapeutic approach for endothelial dysfunction present limited antithrombogenic potentials However few attempts have been done to improve the antithrombogenic potentials of EPCs Our previous study proved that in vitro shear stress contributes to the increase in tPA production by human EPCs Here we further investigated whether in vitro shear stress contributes to the secretion of antithrombogenic substances including plasminogen activator inhibitor1 PAI1 and prostaglandin I2 PGI2 by human EPCsThe peripheral blood mononuclear cells of healthy subjects were induced into EPCs Then the human EPCs were treated with four levels of shear stress including stationary condition low media and high flow shear stress The production of PAI1 and 6ketoprostaglandin F11α6KetoPGF1α by human EPCs with shear stress treatment were measuredIn vitro medium and high flow shear stress inhibited PAI1 secretion by human EPCs but low flow shear stress had no effect on PAI1 secretion by human EPCs All levels of shear stress significantly increased 6KetoPGF1α production by human EPCs in a dosedependent manner
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