The expression of mammalian target of rapamycin in

Authors: Xiaomao Li Lan Xiao Yuebo Yang Huimin Shen Haitao Zeng Zehua Wang

Publish Date: 2008/06/19

Volume: 28, Issue: 3, Pages: 340-342

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Abstract

The activation of mammalian target of rapamycin mTOR signaling pathway in endometrial carcinoma cells Ishikawa and HEC1A was investigated The expression of mTOR was detected by confocal fluorescence microscopy in Ishikawa and HEC1A cells The mRNA levels of PTEN and mTOR the downstream substrate S6K1 and 4EBP1 protein were assayed by RTPCR and Western blot respectively The expression of PTEN in Ishikawa cells was deficient but intact in HEC1A cells respectively P001 There was mTOR expression in both Ishikawa and HEC1A cells and the phosporylated substrate levels in Ishikawa cells were higher than those in HEC1A cells P005 mTOR signaling pathway is activated in two endometrial carcinoma cell strains and the status of activation is related with PTEN expression of the cells The activation level of mTOR is higher in PTENdeficient endometrial carcinoma cells than that in PTENintact endometrial carcinoma cells

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