Somatic embryogenesis and histological analysis fr

Authors: Hui Li Fenglan Li Jingchuan Du Hai Lu Zhengquan He

Publish Date: 2008/12/04

Volume: 10, Issue: 4, Pages: 253-

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Abstract

We examined the somatic embryogenesis from and histological studies of zygotic embryos of seeds in European Grape ‘Moldova’ Vitis vinifera L ‘Moldova’ Primary calli were initiated on Nitsch and Nitsch NN medium supplemented with 10 mg·L−1 24D and 05 mg·L−1 6BA Embryogenic calli were produced upon transfer to a NN medium with 05 mg·L−1 6BA and 2 mg·L−1 NAA and somatic embryos were obtained on a half strength MS medium without plant growth regulators During the somatic embryo germination an addition of 10 mg·L−1 6BA in the medium could accelerate somatic embryos to develop into normal plants and increase the conversion rate from 0 to 433 Histological studies of embryogenic calli and somatic embryos demonstrated dynamic changes of proteins and starch grains The developmental processes of somatic embryos were similar to those of zygotic embryos including typical epiderma cotyledon primordium and vascular tissue

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