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Title of Journal: Bioenerg Res

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Abbravation: BioEnergy Research

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Springer-Verlag

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DOI

10.1007/bf02295280

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1939-1242

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Solutionstate 2D NMR of Ballmilled Plant Cell Wa

Authors: Hoon Kim John Ralph Takuya Akiyama
Publish Date: 2008/03/04
Volume: 1, Issue: 1, Pages: 56-66
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Abstract

Although finely divided ballmilled whole cell walls do not completely dissolve in dimethylsulfoxide DMSO they readily swell producing a gel Solutionstate twodimensional 2D nuclear magnetic resonance NMR of this gel produced directly in the NMR tube provides an interpretable structural fingerprint of the polysaccharide and lignin components of the wall without actual solubilization and without structural modification beyond that inflicted by the ball milling and ultrasonication steps Since the cellulose is highly crystalline and difficult to swell the component may be underrepresented in the spectra The method however provides a more rapid method for comparative structural evaluation of plant cell walls than is currently available With the new potential for chemometric analysis using the 2D NMR fingerprint this method may find application as a secondary screen for selecting biomass lines and for optimizing biomass processing and conversion efficiencies


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