Development of Fundamental Technologies for Micro

Authors: Kiichi Sato Takehiko Kitamori

Publish Date: 2009

Volume: , Issue: , Pages: 251-265

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Abstract

This chapter reviews the development of fundamental technologies required for microchipbased bioreactors utilizing living mammalian cells and pressure driven flow The most important factor in the bioreactor is the cell culture For proper cell culturing continuous medium supply from a microfluidic channel and appropriate modification of the channel surface to accommodate cell attachment is required Moreover the medium flow rate should be chosen carefully because shear stress affects cell activity The techniques presented here could be applied to the development of micro bioreactors such as microlivers pigment production by plant cells and artificial insemination

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