Efficacy of bacterial consortiumAIE2 for contempo

Authors: Chirayu Desai Kunal Jain Bharat Patel Datta Madamwar

Publish Date: 2009/06/11

Volume: 20, Issue: 6, Pages: 813-

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Abstract

Bacterial consortiumAIE2 with a capability of contemporaneous CrVI reduction and azo dye RV5 decolourization was developed from industrial wastewaters by enrichment culture technique The 16S rRNA gene based molecular analyses revealed that the consortium bacterial community structure consisted of four bacterial strains namely Alcaligenes sp DMA Bacillus sp DMB Stenotrophomonas sp DMS and Enterococcus sp DME Cumulative mechanism of CrVI reduction by the consortium was determined using in vitro CrVI reduction assays Similarly the complete degradation of Reactive Violet 5 RV5 dye was confirmed by FTIR spectroscopic analysis ConsortiumAIE2 exhibited simultaneous bioremediation efficiencies of 978 ± 14 and 741 ± 12 in treatment of both 50 mg l−1 CrVI and RV5 dye concentrations within 48 h of incubation at pH 7 and 37°C in batch systems Continuous bioreactor systems achieved simultaneous bioremediation efficiencies of 984 ± 15 and 975 ± 14 after the onset of steadystate at 50 mg l−1 input CrVI and 25 mg l−1 input RV5 concentrations respectively at medium dilution rate D of 0014 h−1 The 16S rRNA gene copy numbers in the continuous bioreactor as determined by realtime PCR assay indicated that Alcaligenes sp DMA and Bacillus sp DMB dominated consortium bacterial community during the active continuous bioremediation processWe are thankful to Department of Biotechnology Ministry of Science and Technology India for providing financial grants We thank Dr C J Joshi AAU Gujarat India for kindly extending technical help and facilities for Real TimePCR analysis EndeavourIndia Research Fellowship awarded to Dr Desai by the Australian Government Department of Education Science and Training DEST is gratefully acknowledged

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