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Title of Journal: Photosynth Res

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Abbravation: Photosynthesis Research

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Publisher

Springer Netherlands

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DOI

10.1007/bf01840730

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ISSN

1573-5079

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Widefield photon counting fluorescence lifetime i

Authors: Zdeněk Petrášek HannJörg Eckert Klaus Kemnitz
Publish Date: 2009/06/17
Volume: 102, Issue: 2-3, Pages: 157-
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Abstract

Fluorescence lifetime imaging microscopy FLIM is a technique that visualizes the excited state kinetics of fluorescence molecules with the spatial resolution of a fluorescence microscope We present a scanningless implementation of FLIM based on a time and spacecorrelated single photon counting TSCSPC method employing a positionsensitive quadrant anode detector and widefield illumination The standard timecorrelated photon counting approach leads to picosecond temporal resolution making it possible to resolve complex fluorescence decays This allows parallel acquisition of timeresolved images of biological samples under minimally invasive lowexcitation conditions 10mW/cm2 In this way unwanted photochemical reactions induced by high excitation intensities and distorting the decay kinetics are avoided Comparably low excitation intensities are practically impossible to achieve with a conventional laser scanning microscope where focusing of the excitation beam into a tight spot is required Therefore widefield FLIM permits to study Photosystem II PS II in a way so far not possible with a laser scanning microscope The potential of the widefield TSCSPC method is demonstrated by presenting FLIM measurements of the fluorescence dynamics of photosynthetic systems in living cells of the chlorophyll dcontaining cyanobacterium Acaryochloris marina


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  1. Adaptation of photosystem II to high and low light in wild-type and triazine-resistant Canola plants: analysis by a fluorescence induction algorithm
  2. Significance of molecular crowding in grana membranes of higher plants for light harvesting by photosystem II
  3. Structures and functions of the extrinsic proteins of photosystem II from different species
  4. Crassulacean acid metabolism (CAM) in an epiphytic ant-plant, Myrmecodia beccarii Hook.f. (Rubiaceae)
  5. The diversity and complexity of the cyanobacterial thioredoxin systems
  6. Reconstituted CP29: multicomponent fluorescence decay from an optically homogeneous sample
  7. A photoacoustic method for rapid assessment of temperature effects on photosynthesis
  8. Photosynthesis-related quantities for education and modeling
  9. Temperature responses of the Rubisco maximum carboxylase activity across domains of life: phylogenetic signals, trade-offs, and importance for carbon gain
  10. H-transfers in Photosystem II: what can we learn from recent lessons in the enzyme community?
  11. Molecular signatures for the main phyla of photosynthetic bacteria and their subgroups
  12. Optimization of photosynthesis by multiple metabolic pathways involving interorganelle interactions: resource sharing and ROS maintenance as the bases
  13. Production of reactive oxygen species in decoupled, Ca 2+ -depleted PSII and their use in assigning a function to chloride on both sides of PSII
  14. Tracking the molecular evolution of photosynthesis through characterization of atomic contents of the photosynthetic units
  15. What governs the reaction center excitation wavelength of photosystems I and II?
  16. Fluorescence spectroscopy of reconstituted peridinin–chlorophyll–protein complexes
  17. Photosynthetic carbon acquisition in Sargassum henslowianum (Fucales, Phaeophyta), with special reference to the comparison between the vegetative and reproductive tissues
  18. Photosynthesis Web resources
  19. Thioredoxin: an unexpected meeting place
  20. Michael Cusanovich: a man of many talents and interests
  21. Phylogeny and taxonomy of Chlorobiaceae
  22. The biological and geological contingencies for the rise of oxygen on Earth
  23. The photoenzymatic cycle of NADPH: protochlorophyllide oxidoreductase in primary bean leaves ( Phaseolus vulgaris ) during the first days of photoperiodic growth
  24. Carbon allocation and element composition in four Chlamydomonas mutants defective in genes related to the CO 2 concentrating mechanism
  25. Comparison of oligomeric states and polypeptide compositions of fucoxanthin chlorophyll a / c -binding protein complexes among various diatom species
  26. Iron–sulfur cluster biosynthesis in photosynthetic organisms
  27. A chloroplast pump model for the CO 2 concentrating mechanism in the diatom Phaeodactylum tricornutum

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