Simvastatin in contrast to postconditioning reduce

Authors: Efstathios K Iliodromitis Ioanna Andreadou Eftihios Prokovas Anastasia Zoga Dimitrios Farmakis Theano Fotopoulou Konstantinos Ioannidis Ioannis A Paraskevaidis Dimitrios Th Kremastinos

Publish Date: 2010/01/12

Volume: 105, Issue: 2, Pages: 193-203

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Abstract

Postconditioning POC reduces lethal reperfusion injury under normal conditions but its effectiveness under certain pathological states is in dispute In the present study we sought to determine the effect of chronic simvastatin treatment in hyperlipidemic animals with or without POC Anesthetized rabbits were randomized into eight groups as follows and were subjected to 30min myocardial ischemia followed by 3h reperfusion Normally fed animals a Control group with no additional intervention a Sim group treated with simvastatin for 3 weeks at a dose of 3 mg kg−1 a POC group subjected to POC with eight cycles of 30s ischemia/reperfusion a SimPOC group treated with simvastatin and POC Cholesterol fed 6 weeks animals a Chol group with no additional interventions a Chol–Sim group treated with simvastatin for 3 weeks a CholPOC group subjected to POC and a Chol–SimPOC group treated with simvastatin and POC Infarct size and plasma levels of malondialdehyde MDA nitrotyrosine NT NO x total cholesterol and LDL were evaluated In a second series of experiments heart tissue samples were taken for MDA NT and NO x assessment Infarct size circulating MDA NT NO x and cardiac MDA NT and NO x levels declined in POC and all Sim groups compared with Control Chol and CholPOC p  005 Simvastatin also reduced total cholesterol and LDL plasma levels In conclusion a 3week simvastatin treatment limits the infarct size and attenuates the oxidative and nitrosative stress both in normo and in hypercholesterolemic rabbits subjected to ischemia–reperfusion irrespective of the presence of POC while POC is effective only in normocholesterolemic animals

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