Body weight and energy homeostasis was not affecte

Authors: Anne Noatsch Klaus J Petzke Marion K Millrose Susanne Klaus

Publish Date: 2010/12/18

Volume: 50, Issue: 6, Pages: 479-488

PDF Link

Abstract

The aim of this study was to assess and to compare the effects of highprotein diets with dietary leucine supplementation in mice particularly on energy homeostasis body composition and expression of uncoupling protein UCP which are suggested to decrease food energy efficiencyMale C57BL/6 mice were exposed for 14 weeks to semisynthetic diets containing either 20 adequate protein content AP or 50 whey protein highprotein content HP A third group was fed the AP diet supplemented with Lleucine AP + L corresponding to the leucine content of the HP diet The total fat content was 5 w/wBody weight gain body composition energy expenditure and protein expression of UCP1 in brown adipose tissue and UCP3 in skeletal muscle were not different between groups In HPfed mice a stronger increase in blood glucose levels was detected during glucose tolerance tests compared to AP and AP + L whereas plasma insulin was similar in all groups Leucine supplementation did not affect glucose tolerance Plasma cholesterol was significantly decreased in HP and AP + L when compared to AP Plasma triglyceride concentrations were increased twofold in HPfed mice when compared to AP + L and AP groups Liver and skeletal muscle triglyceride and glycogen concentrations were similar in all groups Postabsorptive plasma concentrations of branchedchain amino acids were not significantly increased after exposure to HP and AP + L diets whereas those of lysine were decreased in HP and AP + L mice when compared to AP P  0001 Plasma methionine concentrations were lower after HP intake when compared to AP and AP + L P  005We suggest that an exposure of mice to HP diets or a corresponding leucine supplementation has no significant effect on energy homeostasis and UCP expression compared with AP diets when feeding a lowfat diet The use of highquality whey protein might at least in part explain the results obtained

Keywords: