Journal Title
Title of Journal: Diabetol Notes Lect
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Abbravation: Diabetologia Notes de lecture
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Publisher
Springer-Verlag
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Authors: Laurence Kessler
Publish Date: 2010/02/05
Volume: 2, Issue: 1, Pages: 13-14
Abstract
The proximity of endothelial cells and beta cells in islets by necessity means that they are exposed to each other’s products Whereas islet endothelial cells require signals from beta cells to function properly endothelin1 thrombospondin1 and laminins among others have been identified as endothelialderived molecules although their full effects on beta cells have not been explored We tested the hypothesis that islet endothelialderived products can affect the function of beta cellsEndothelial cells from rat islets were proliferated and purified Endotheliumconditioned culture medium ECCM was obtained by maintaining the endothelial cells in culture medium Islet function was evaluated by following the exposure of cultured islets to standard culture medium or ECCM Changes in mRNA levels for key beta cell metabolic enzymes were also measured in islets after ECCM exposureGlucosestimulated insulin release and islet insulin content were markedly enhanced by exposure to ECCM This was at least partly explained by improved mitochondrial function assessed by glucose oxidation and an upregulation of the mitochondrial gene for glycerol3phosphate dehydrogenase mGpdh also known as Gpd2 combined with upregulation of the ratelimiting enzyme in the glycolysis glucokinase in the islets The intracellular degradation of insulin was also decreased in the islets Islet endothelial cells produced laminins and the positive effects of islet endothelial cells were prevented by addition of a neutralising antibody to the β1chain of laminin Addition of exogenous laminin stimulated islet function
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