Quantitative comparison of the expression of myoge

Authors: Yuqing Zhang Xungang Tan Peng Xu Wei Sun Yongli Xu Peijun Zhang

Publish Date: 2010/03/20

Volume: 28, Issue: 2, Pages: 248-253

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Abstract

MyoD Myf5 and myogenin are myogenic regulatory factors that play important roles during myogenesis It is thought that MyoD and Myf5 are required for myogenic determination while myogenin is important for terminal differentiation and lineage maintenance To better understand the function of myogenic regulatory factors in muscle development of flounder an important economic fish in Asia realtime quantitative RTPCR was used to characterize the expression patterns of MyoD Myf5 and myogenin at early stages of embryo development and in different tissues of the adult flounder The results show that Myf5 is the first gene to be expressed during the early stages of flounder development followed by MyoD and myogenin The expressions of Myf5 yoD and myogenin at the early stages have a common characteristic expression gradually increased to a peak level and then gradually decreased to an extremely low level In the adult flounder the expression of the three genes in muscle is much higher than that in other tissues indicating that they are important for muscle growth and maintenance of grown fish During embryonic stages the expression level of MyoD might serve an important role in the balance between muscle cell differentiation and proliferation When the MyoD expression is over 30 of its highest level the muscle cells enter the differentiation stageSupported by the Key Laboratory of Experimental Marine Biology Academia Sinica Qingdao China the National High Technology Research and Development Program of China 863 Program No 2006AA10AA402 the National Basic Research Program of China 973 Program Nos 2004CB117402 2010CB126304 and the National Natural Science Foundation of China No 30871929

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