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Authors: Hanbing Zhong Shuo Lin
Publish Date: 2011
Volume: , Issue: , Pages: 193-205
Abstract
Functional chemicals are very useful tools for molecular biology studies Due to its small size large progeny clutch and embryonic transparency zebrafish serves as a superb in vivo animal model for chemical compound screens and characterization During zebrafish embryogenesis multiple developmental phenotypes can be easily examined under the microscope therefore allowing a more comprehensive evaluation for identifying novel functional chemicals than cellbased assays Ever since the first zebrafishbased chemical screen was conducted in the year 2000 many functional chemicals have been discovered using this strategy In this chapter we describe how to perform a typical zebrafishbased chemical screen and discuss the details of the protocol by using the example of the identification and characterization of two new Smo inhibitors with a GliGFP transgenic line
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