Manufacturing of AdenoAssociated Viruses for Exa

Authors: Haifeng Chen

Publish Date: 2011

Volume: , Issue: , Pages: 235-246

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Abstract

Adenoassociated virus AAV is one of the most promising vectors for gene therapy There are several ways of producing AAV vectors but largescale production of this vector remains a major challenge Virovek developed a novel method of expressing the AAV Rep and Cap genes in insect cells mediated by intronsplicing mechanism and producing AAV vectors with these Rep and Cap sequences containing the artificial intron The recombinant baculoviruses harboring these artificial introncontaining Rep and Cap sequences are very stable and the AAV vectors produced in insect cells with these recombinant baculoviruses are very infectious

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