Digital Image Capture for Confocal Microscopy

Authors: W Gray Jay Jerome

Publish Date: 2011

Volume: , Issue: , Pages: 133-155

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Abstract

Good microscopy includes optimizing the information obtainable from your confocal image with the minimum amount of effort and time A key to achieving this goal is to match as closely as possible the parameters of all hardware and software in the image collection train For reasons outlined later in this chapter it is particularly important to match the resolution of your digital image to the output resolution from the microscope In we discussed the resolution parameters of the digital image In this chapter we cover the optical resolution obtainable from fluorescence confocal light microscopes we review the important parameters of the image acquisition hardware that influence image information and we illustrate how to match these two parameters to optimize confocal image acquisition

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