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Title of Journal: BioControl

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Abbravation: BioControl

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Springer Netherlands

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DOI

10.1016/j.ahj.2004.12.017

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1573-8248

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Specific SCAR markers and multiplex realtime PCR

Authors: Xin Mei Feng AnnaIda Johnsson Holmberg Ingvar Sundh Thomas Ricard Petter Melin
Publish Date: 2011/05/05
Volume: 56, Issue: 6, Pages: 903-913
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Abstract

Several strains from the genus Trichoderma Ascomycetes Hypocreales are commercially used as biocontrol agents eg in formulations containing the two Trichoderma strains IMI206039 Hypocrea parapilulifera BS Lu Druzhinina Samuels and IMI206040 T atroviride P Karst To quantify the presence of the two isolates after application we developed primers for SCAR markers SequenceCharacterised Amplified Region In order to quantify both fungal strains simultaneously we also designed fluorophorelabelled probes distinguishing the two strains to be used in combination with the SCAR primers In incubations of two different soils artificially inoculated and maintained under controlled conditions the quantification through amplification with the SCAR markers in qPCR and through colonyforming units from plate counting correlated well Further tests of the markers on samples taken from a golf green treated with a product containing both strains indicated that the two biocontrol strains did not establish either on the golf green or in the surrounding areaThe authors would like to thank Per Hansson greenkeeper at the St Arild golf course for letting us taking golf green samples and Leticia Pizzul for supplying the Ultuna soil and giving practical suggestions for soil treatment in the laboratory This work was largely funded by MISTRA Foundation for Strategic Environmental Research with additional support from BINAB BioInnovation AB

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  2. Interactions between the parasitoid wasp Dinocampus coccinellae and two species of coccinellid from Japan and Britain
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  10. Field study demonstrates that exotic parasitoids (Hymenoptera: Encyrtidae) of mealybugs (Hemiptera: Pseudococcidae) are absent from a native forest habitat in New Zealand
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