Assessment of the presence of extendedspectrum be

Authors: P Egea L LópezCerero M D Navarro J RodríguezBaño A Pascual

Publish Date: 2011/07/14

Volume: 30, Issue: 9, Pages: 1045-

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Abstract

The principal objective of this study was to assess whether chicken eggshells may be contaminated by ESBLproducing E coli ESBLC Additional analyses were carried out to determine if ESBLEC could be detected in other foodstuffs such as cooked poultry or fresh vegetables Seventytwo eggshells from different supermarkets and stores as well as 32 salads 30 samples of cooked poultry and six samples of chickenbased pet food samples were analysed Characterization of ESBL was performed by PCR and sequencing Antimicrobial resistance was determined by disk diffusion method Phylogenetic group was assigned by multiplexPCR No ESBLEC was isolated from chicken eggshells cooked chicken and pet food One SHV12producing E coli was isolated from a salad sample This is the first study to analyse chicken eggs in an area where there is a high prevalence of ESBLEC in retail chicken meatThis study was supported by the Ministerio de Sanidad y Consumo Instituto de Salud Carlos III FEDER Spanish Network for Research into Infectious Diseases REIPI RD06/0008 Fondo de Investigación Sanitaria FIS Instituto de Salud Carlos III PI070190 Consejerías de Salud e Innovación Junta de Andalucía PI0048/2008 and CTS5259 and the Ministerio de Educación y Ciencia AGL200802129 We thank Beatriz Muñoz Hurtado for her assistance in European and Spanish legislation

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