Authors: Nicholas Pugh Gavin E Jarvis Annelize Koch Kjell S Sakariassen Bill Davis Richard W Farndale
Publish Date: 2011/11/27
Volume: 33, Issue: 1, Pages: 6-15
Abstract
This study was designed to assess the effect of Factor Xa antagonists on thrombus formation at various axial positions on a tissue factor rich surface under arterial blood flow conditions Nonanticoagulated flowing human blood drawn directly from an antecubital vein was perfused over a tissue factor coated cover slip in a parallelplate perfusion chamber Thrombus surface coverage thrombus mean height and fibrin surface coverage were measured at six different axial positions by confocal microscopy Both thrombus surface coverage and mean height decreased along the cover slip axis whereas the fibrin surface coverage increased Prechamber treatment of blood with the direct Factor Xa inhibitors Razaxaban and 813893 resulted in significantly reduced thrombus and fibrin formation at all axial positions investigated P 005 Thrombus and fibrin deposition in a laminar flow chamber changed with axial position with surface coverage measurements being more reproducible than thrombus mean height Data were more reproducible towards the centre of the flow chamber than at the extremities Razaxaban and 813893 inhibited thrombus and fibrin formation at the highest concentrations tested No difference in drug effect was apparent at different axial positions In conclusion axial position influences the degree of thrombus and fibrin deposition with measurements being less reproducible at the extremities of the flow chamber This technique may prove useful for analysing antithrombotic drug effects before progression to clinical trials
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