Role of transforming growth factorβ1 in the proce

Authors: Meng Fanbin Chen Jianghai Liu Juan Wang Yang Weng Yuxiong Chen Yanhua Li Tao Chen Zhenbing

Publish Date: 2011/02/19

Volume: 31, Issue: 1, Pages: 77-82

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Abstract

In order to investigate the biological function of transforming growth factorβ1 TGFβ1 during fibrosis in denervated skeletal muscle we recruited sciatic nerve injury model of SD rats in which denervated gastrocnemius was isolated for analysis At different time points after operation denervated muscle was examined by several methods Masson trichrome staining showed morphological changes of denervated skeletal muscle Quantitative RTPCR detected the rapid increase of TGFβ1 expression at mRNA level after nerve injury It was found that a peak of TGFβ1 mRNA expression appeared one week postoperation The expression of collagen I COL I mRNA was upregulated in the nerve injury model as well and reached highest level two weeks postinjury Immunoblot revealed similar expression pattern of TGFβ1 and COL I in denervated muscles at protein level In addition we found that the area of the gastrocnemius muscle fiber was decreased gradually along with increased interstitital fibrosis Interestingly this pathological change could be prevented at least partly by local injection of TGFβ1 antibodies which could be contributed to the reduced production of COL I by inhibiting function of TGFβ1 Taken together in this study we demonstrated that the expression of TGFβ1 was increased significantly in denervated skeletal muscle which might play a crucial role during muscle fibrosis after nerve transection

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