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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme

Authors: Xinyi Wang, Yao Xu, Yang Chen, Limei Li, Feng Liu, Na Li,

Publish Date: 2011/04/05
Volume: 400, Issue:7, Pages: 2085-2091
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We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering (PRLS) measurement and naked-eye detection using for the first time the lysozyme DNA aptamer as the recognition element. Lysozyme DNA aptamer could stabilize gold nanoparticles (AuNPs) at high ionic strength. Introducing lysozyme to the system easily triggered the aggregation of AuNPs, producing a red-to-blue color change of the solution, red-shifted plasmon absorption, and enhanced plasmon resonance light scattering. The linear range was found to be 0.2∼4 nM for 0.7 nM AuNPs, 0.3∼6 nM for 1.4 nM AuNPs and 0.6∼8 nM for 2.1 nM AuNPs. About 0.1 nM lysozyme can produce an observable enhancement of PRLS signal. For visual detection, 1 nM lysozyme can produce a very distinctive color change. Satisfactory recoveries were obtained for simulated saliva and diluted urine samples, indicating that the method has potential for analyses of clinical samples. The simplicity and high sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine analysis.



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Other Papers In This Journal:

  1. Spectroscopic and voltammetric studies of Pefloxacin bound to calf thymus double-stranded DNA
  2. Study of normal colorectal tissue by FT-Raman spectroscopy
  3. Portable Raman versus portable mid-FTIR reflectance instruments to monitor synthetic treatments used for the conservation of monument surfaces
  4. Quantification of urinary AICAR concentrations as a matter of doping controls
  5. Development and application of molecularly imprinted polymers as solid-phase sorbents for erythromycin extraction
  6. Downscaling the in vitro test of fungal bioremediation of polycyclic aromatic hydrocarbons: methodological approach
  7. Evalution of sequential extractions on dry and wet sediments
  8. Comparison of CIM discs and CPG glass as solid supports for bioanalytical columns used in allergen detection
  9. An LCMS method for the assay of melittin in cosmetic formulations containing bee venom
  10. A novel 1
  11. Tip-enhanced near-field optical microscopy of carbon nanotubes
  12. Characterizing single crystal surfaces using high resolution electron diffraction
  13. Micro-perforated elastomeric poly(dimethylsiloxane) mask fabricated using high-aspect-ratio micro-pillar arrays for spatially defined surface modification: an unconventional method for establishing a microarray platform
  14. Analytical investigations of tunnel magnetoresistance layers
  15. Metabolic profiling reveals new serum biomarkers for differentiating diabetic nephropathy
  16. Fluorescence polarization immunoassay using IgY antibodies for detection of valnemulin in swine tissue
  17. Morphology and efficiency of poly(styrene-co-divinylbenzene)-based monolithic capillary columns for the separation of small and large molecules
  18. Suspect screening and target quantification of multi-class pharmaceuticals in surface water based on large-volume injection liquid chromatography and time-of-flight mass spectrometry
  19. Solution-phase detection of dual microRNA biomarkers in serum
  20. Continuous measurement of metals in flue gas using ICP-OES
  21. Estimation of environmental mobility of heavy metals using a sequential leaching of particulate material emitted from an opencast chrome mine complex
  22. Rapid urinary tract infection diagnostics by surface-enhanced Raman spectroscopy (SERS): identification and antibiotic susceptibilities
  23. A general approach for the purification and quantitative glycomic analysis of human plasma
  24. Mass spectrometry based phospholipidomics of mammalian thymus and leukemia patients: implication for function of iNKT cells
  25. High-performance liquid chromatographic determination of cocaine and benzoylecgonine by direct injection of human blood plasma sample into an alkyl-diol-silica (ADS) precolumn
  26. Dynamic monitoring of glucagon secretion from living cells on a microfluidic chip
  27. Passive sampling and stir bar sorptive extraction for the determination of endocrine-disrupting compounds in water by GC-MS
  28. Detection of discoloration in diesel fuel based on gas chromatographic fingerprints
  29. Detection of bacteria by surface-enhanced Raman spectroscopy
  30. Barbell-shaped stir bar sorptive extraction using dummy template molecularly imprinted polymer coatings for analysis of bisphenol A in water
  31. Precision of Raman depolarization and optical attenuation measurements of sound tooth enamel
  32. Pathogen detection using engineered bacteriophages
  33. Structural study of spirolide marine toxins by mass spectrometry
  34. A clinical trial for therapeutic drug monitoring using microchip-based fluorescence polarization immunoassay
  35. Problems in obtaining precise and accurate Sr isotope analysis from geological materials using laser ablation MC-ICPMS
  36. High-throughput analysis of therapeutic and diagnostic monoclonal antibodies by multicapillary SDS gel electrophoresis in conjunction with covalent fluorescent labeling
  37. Characterization of polyoxyethylenes according to the number of hydroxy end groups by hydrophilic interaction chromatography at critical conditions for polyethylene glycol
  38. Micro-Raman analysis of coloured lithographs
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  40. High-throughput carotenoid profiling using multivariate curve resolution
  41. Detection of chemical agents in the atmosphere by open-path FT-IR spectroscopy under conditions of background interference: II. Fog and rain
  42. PEGylated polyethyleneimine grafted silica nanoparticles: enhanced cellular uptake and efficient siRNA delivery
  43. Wide mass range trapping using a 7-T internal source matrix-assisted laser desorption/ionization Fourier transform mass spectrometer
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  45. Analysis of flame retardants and elements of concern in printed wiring boards with respect to origin and year of construction
  46. Study of OSEM with different subsets in grating-based X-ray differential phase-contrast imaging
  47. Preparation and certification of arsenobetaine reference material NMIJ CRM 7901-a
  48. Characterization of a mixed-mode reversed-phase/cation-exchange stationary phase prepared by thermal immobilization of poly(dimethylsiloxane) onto the surface of silica
  49. Aequorin mutants with increased thermostability
  50. The potential of asymmetric flow field-flow fractionation hyphenated to multiple detectors for the quantification and size estimation of silica nanoparticles in a food matrix
  51. Micro free-flow electrophoresis: theory and applications
  52. Determination of perfluorinated alkyl acid concentrations in biological standard reference materials
  53. Thermal preparation of lysozyme-imprinted microspheres by using ionic liquid as a stabilizer
  54. Development and applications of whole cell biosensors for ecotoxicity testing
  55. Role of the activity coefficient in the dissemination of pH: comparison of primary (Harned cell) and secondary (glass electrode) measurements on phosphate buffer considering activity and concentration scales
  56. Cross validation of multiple methods for measuring pyrethroid and pyrethrum insecticide metabolites in human urine
  57. A high throughput glucocerebrosidase assay using the natural substrate glucosylceramide
  58. The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme
  59. LC-APCI-MS-MS methodology for determination of glybenclamide in human plasma

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