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Humana Press

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Different Strategies for Hyperactivation of Lipase

Authors: Jose M Palomo Jose M Guisan
Publish Date: 2012
Volume: , Issue: , Pages: 329-341
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Abstract

One important parameter for the application of lipase catalysts in chemical industries is the specific activity displayed towards natural or unnatural substrates Different strategies to enhance the lipase activity have been described The immobilization of lipases on hydrophobic supports by interfacial adsorption at low ionic strength permitted the hyperactivation of these enzymes by fixing the open conformation of the lipase on the hydrophobic support Improvements of activity from 12 up to 20fold with respect to the initial one have been observed for lipases from different sourcesA second strategy was based on the presence of additives in particular surfactants with hydrophobic character to enhance the activity of lipases immobilized on macroporous supports up to 8 fold and even more than 100fold in some cases for soluble lipases


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