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Authors: Jason Rice Myron Christodoulides
Publish Date: 2012
Volume: , Issue: , Pages: 405-419
Abstract
DNAbased vaccines are currently being developed for treating a diversity of human diseases including cancers autoimmune conditions allergies and microbial infections In this chapter we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogenderived antigens using Neisseria meningitidis as an example In addition we describe a fusion genebased vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogenderived polypeptides Finally we provide a safe and effective protocol for delivery of DNA vaccines based on intramuscular injection followed by electroporation
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