Calcium Handling in hiPSCDerived Cardiomyocytes

Authors: Lee YeeKi Siu ChungWah

Publish Date: 2012

Volume: , Issue: , Pages: 1-47

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Abstract

Calcium is crucial in governing the contractile activities of myofilaments in cardiomyocytes The characterization of calcium handling properties in human induced pluripotent stem cell–derived cardiomyocytes iPSCCMCs is of significant interest and pertinent to the stem cell and cardiac regenerative field because of their potential patientspecific therapeutic use In this book readers can learn the approaches and parameters that have to be considered for calcium handling studies these include the importance of the sarcoplasmic reticulum SR which governs the maturity of cardiomyocytes the role of SR junctional proteins that facilitate calcium release and uptake through the SR and the extent of immaturity of iPSCCMCs compared with those derived from human embryonic stem cells in terms of calcium homeostasis Future study would suggest potential uses of hiPSCCMCs as a platform for cardiomyopathy disease modeling especially certain cardiac defects related to impaired calcium homeostasis causing arrhythmia and pumping defeats Last a detailed protocol for calciumsensitive dye calibration calcium transient recording and electrical contraction coupling is included

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