Evaluation of somaclonal variation for genetic imp

Authors: Nagawara Seshagirirao Ravindra Srinivas Iyer Ramesh Mahesh Kumar Gupta Tripta Jhang Ashutosh Kumar Shukla Mahendra Pandurang Darokar Raghavendra Narayanrao Kulkarni

Publish Date: 2012/04/12

Volume: 15, Issue: 1, Pages: 33-39

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Abstract

Patchouli Pogostemon patchouli is an important exclusively vegetatively propagated aromatic plant whose essential oil is widely used in perfumery and cosmetic products Forty SC1 generation first generation following in vitro phase somaclones selected randomly from about 400 somaclones developed from the variety Johore were multiplied through stem cuttings and evaluated in SC2 and SC3 generations to study the extent of somaclonal variation generated for plant height herb yield essential oil content essential oil yield and seven constituents of the essential oil Significant or highly significant somaclonal variation was observed for plant height herb yield essential oil content essential oil yield and contents of patchouli alcohol αguaiene αδpatchoulene and αbulnesene in the essential oil The number of somaclones significantly superior to the parental variety for plant height herb yield essential oil content and patchouli alcohol content in the essential oil ranged from 8–16 and the maximum superiority over the parental variety for these traits ranged from 21–79 Broadsense heritability estimates of plant height herb yield and essential oil content were 060–070 while those of essential oil yield and patchouli alcohol content were 044 and 047 respectively Heritability estimates of other studied essential oil constituents were generally low 012–038 A high positive correlation was observed between essential oil yield and herb yield suggesting that selection for herb yield would be effective in improving essential oil yield Patchouli alcohol content in the essential oil was negatively correlated with all the studied traits Somaclonal variation heritabilities of traits and intertrait correlations are reported for the first time in patchouli

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