Astilbin inhibits proliferation of rat aortic smoo

Authors: Ping Li Sihai Gao Wei Jie Qilin Ao Yafei Huang

Publish Date: 2012/04/20

Volume: 32, Issue: 2, Pages: 181-185

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Abstract

This study examined the effect of astilbin on the proliferation of rat aortic smooth muscle cells RASMCs induced by angiotensin II AngII and explored the possible mechanisms Cell proliferation model of RASMCs was induced by treatmente with AngII Cells were randomly divided to 8 groups Normally cultured VSMCs serves as blank control group in AngII model group cells were treated with AngII at 10−7 mol/L in three astilbin groups cells were treated with 10 15 30 mg/L of astilbin in three AngII+astilbin groups cells were treated with AngII at 10−7 mol/L and astilbin at 10 15 30 mg/L Cell proliferation ability was detected by MTT method and the cell cycles and proliferation index were flow cytometrically determined The expression of cmyc mRNA was assessed by using reverse transcription polymerase chain reaction RTPCR and the expression of NFκB in RASMCs was immunocytochemically observed Our results showed that MTT metabolism in RASMCs in the basic and AngII stimulated situation was inhibited by astilbin and the cells numbers of G0/G1 phase were increased and that of G2/S phase were decreased markedly Not only highly expression of cmyc gene stimulated by AngII could be inhibited by Astilbin significantly but also the expression of NFκB protein can be down regulated by Astilbin We are led to conclude that astilbin astilbin can inhibit the AngIImediated proliferation of RASMCs by blocking the transition of RASMCs from G0/G1 phase to S phase and by downregulating the expression of NFκB cmyc gene

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