A kinetic approach to assess oxidative metabolism

Authors: Paula Mariela González Doris Abele Susana Puntarulo

Publish Date: 2012/07/25

Volume: 131, Issue: 4, Pages: 253-264

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Abstract

Electron paramagnetic resonance uses the resonant microwave radiation absorption of paramagnetic substances to detect highly reactive and therefore shortlived oxygen and nitrogen centered radicals Previously steady state concentrations of nitric oxide ascorbyl radical A· and the labile iron pool LIP were determined in digestive gland of freshly collected animals from the North Sea bivalve Mya arenaria The application of a simple kinetic analysis of these data based on elemental reactions allowed us to estimate the steady state concentrations of superoxide anion the rate of A· disappearance and the content of unsaturated lipids This analysis applied to a marine invertebrate opens the possibility of a mechanistic understanding of the complexity of free radical and LIP interactions in a metabolically slow cold water organism under unstressed conditions This data can be further used as a basis to assess the cellular response to stress in a simple system as the bivalve M arenaria that can then be compared to cells of higher organismsThis study was supported by grants from the University of Buenos Aires National Council for Science and Technology CONICET PIP 1171 and DAADCONICET collaborative visit SP is career investigator from CONICET and PMG is a postdoctoral fellow from CONICET

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