Impact of sodium nitroprusside on nitrate reductas

Authors: Shamsul Hayat Sangeeta Yadav Arif Shafi Wani Mohammad Irfan Mohammad Naseer Alyemini Aqil Ahmad

Publish Date: 2012/11/09

Volume: 53, Issue: 5, Pages: 362-367

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Abstract

The present study was carried out to elucidate the protective role of sodium nitroprusside SNP against salt NaCl stress given as seed soaking in tomato Solanum lycopersicum Mill cv K21 For this experiment prior to sowing the surface sterilized tomato seeds were soaked in different concentrations of NaCl solution 50 100 and 150 mM for 8 hours Some NaCl soaked seeds were then transferred to the 10−5 M of SNP solution for 8 hours The plants were sampled at 60 DAS to assess the nitrate reductase activity proline content and the activities of antioxidant enzymes The antioxidant enzyme activity and proline content increased in the plants where SNP was applied as a follow up treatment of the NaCl as seed soaking over the control plants thereby providing stress tolerance to the plants In case of the nitrate reductase activity the toxic effects generated by the lowest concentration of NaCl 50 mM was completely neutralized by SNP and partly at higher concentrations 100/150 mM These results suggest that nitric oxide can be used as a stress alleviator in plants which are grown in the soil contaminated with salinity stress

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