Seroprevalence and Molecular Characterisation of H

Authors: Hakima GharbiKhelifi Nabil Ben Salem Abid Abir Beji Leila Bhiri Rafik Harrath Khira Sdiri Sylviane Billaudel Virginie Ferre Mahjoub Aouni

Publish Date: 2012/03/25

Volume: 23, Issue: 1, Pages: 29-35

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Abstract

The aim of the present study was to investigate the seroprevalence of Hepatitis A virus antibodies in patients with clinical symptoms of viral hepatitis and molecular characterization of the detected isolates The present study deals with the seroprevalence and the genetic diversity of HAV in 400 Tunisian patients presenting in dispensaries 160 patients and in University Hospitals 240 patients with hepatitis symptoms between 2006 and 2008 The patients with acute hepatitis were mainly from rural regions However the total number of patients was decreased over time The collected samples were from patients with hepatitis symptoms occurring mainly during January–March 367 26 and 355 and September–December 394 434 and 355 during the three years of study respectively However HAV infection was established for only 110 among 400 patients The detected isolates were clustered within subgenotype IA The present study constituted another report of the continued surveillance of HAV infection in the region of Monastir and the molecular characterisation of the detected strainsNucleotide sequence data reported are available in the GenBank databases under the accession numbers GQ453437 GQ453438 GQ453439 GQ453440 GQ453441 GQ453442 GQ453443 GQ453444 GQ453445 GQ453446 GQ453447 GQ453448 GQ453449 GQ453450 GQ453451 GQ453452 GQ453453 GQ453454 GQ453455 GQ453456 GQ453457 GQ453458 GQ453459 GQ453460 and GQ453461The authors are grateful to all technician staff of the Laboratory of Transmissible Diseases and Biological Active Substances Faculty of Pharmacy Monastir Tunisia and Department of Microbiology Faculty of Pharmacy Nantes France for their help and support

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