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Publisher
Springer, Berlin, Heidelberg
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Authors: Johann P Klare HeinzJürgen Steinhoff
Publish Date: 2013
Volume: , Issue: , Pages: 205-248
Abstract
Sitedirected spin labelling SDSL in combination with electron paramagnetic resonance EPR spectroscopy is a powerful tool for the investigation of the structure and conformational dynamics of biomolecules including membrane proteins under nativelike conditions EPR spectroscopy of the spinlabelled molecules provides information about the spin label side chain mobility its solvent accessibility the polarity of its immediate environment and intra or intermolecular distances to another paramagnetic centre or spin label This chapter provides an overview of the basics as well as recent progress in SDSL and related EPR techniques Continuous wave EPR spectra analyses and pulse EPR techniques are reviewed with special emphasis on applications to the membraneembedded sensory rhodopsin–transducer complex mediating the photophobic response of the halophilic archaeum Natronomonas pharaonis the maltose ABC importer MalFGK2 and the mechanosensitive channel MscS
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