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Title of Journal: Biol Trace Elem Res

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Abbravation: Biological Trace Element Research

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Humana Press Inc

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DOI

10.1016/j.aprim.2015.01.004

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1559-0720

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Determination of Lead in Biological Samples of Children with Different Physiological Consequences Using Cloud Point Extraction Method

Authors: Faheem Shah, Tasneem Gul Kazi, Naeem Ullah, Hassan Imran Afridi,

Publish Date: 2013/04/28
Volume: 153, Issue:1-3, Pages: 134-140
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Abstract

In present study, lead (Pb) level in biological samples of children with physiological disorders (liver, bone, and gastrointestinal; age ranged 1–10 years) have been assessed. For comparison purpose, age-matched healthy children were also selected. Cloud point extraction (CPE) was employed for preconcentration of Pb in acid-digested biological samples prior to its determination by flame atomic absorption spectrometry (FAAS). Dithizone (diphenylthiocarbazone) and nonionic surfactant Triton X-114 (TX-114) were used as complexing reagent and extractant, respectively. The effects of several experimental variables on proposed CPE were evaluated. Under the optimum experimental conditions, the observed detection limit (LOD) and the enhancement factor (EF) were 0.08 μg L−1 and 53, respectively. Relative standard deviation (RSD) of 10 μg L−1 Pb was 3.4 %. It was observed that children with liver-, bone-, and gastrointestinal-related disorders had three- to fourfold higher Pb level in blood and scalp hair samples.


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