Fluorescence nanoscopy Methods and applications

Authors: Jose RequejoIsidro

Publish Date: 2013/06/04

Volume: 6, Issue: 3, Pages: 97-120

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Abstract

Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional diffractionlimited microscopy This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques labelling strategies to obtain highly detailed superresolved images and in the quantitative methods to extract meaningful information from them

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