Authors: Assaâd Sila Najwa Mlaik Nadhem Sayari Rafik Balti Ali Bougatef
Publish Date: 2013/06/11
Volume: 22, Issue: 1, Pages: 78-87
Abstract
Extraction and depolymerisation of chitin and chitosan from shrimp waste material was carried out using fish proteases aided process A high deproteinization level 80 was recorded with an Enzyme/Substrate ratio of 10 U/mg The demineralization of shrimp waste was completely achieved within 6 h at room temperature in HCl 125 M and the residual content of calcium in chitin was below 001 The degree of Nacetylation calculated from the 13C CP/MASNMR spectrum was 85 The chitin obtained was converted to chitosan by Ndeacetylation Xray diffraction patterns also indicated two characteristics crystalline peaks approximately at 10° and 20° 2θ Chitosan was then evaluated in the treatment of unhairing effluents from the tanning industry A result showed that chitosan as a coagulant has good performance in alkaline pH and at concentration of 05 g/L Within these conditions chitosan could decrease turbidity value total suspended solids 89 at 15 g/L biological oxygen demand 333 at 15 g/L and chemical oxygen demand 587 at 15 g/LThis work is a part of a master by Assaad Sila whose research was supported financially by Ministry of Higher Education and Scientific Research Tunisia through a grant to Laboratoire de Génie Enzymatique et de MicrobiologieENIS We warmly thank Mr Rachid Hajji Unité de Recherche de Service Commun RMN des Solides for his invaluable help with RMN analysis We thank Ms Amira Zineddine Laboratoire Génie des Matériaux et Environnement for his help in the XRay analysis The authors would like to thank Mr Baha Eddine Abdelmalek Shrimp Processing Plant SfaxTunisia for providing of raw materials
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