Journal Title
Title of Journal: Biomol NMR Assign
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Abbravation: Biomolecular NMR Assignments
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Publisher
Springer Netherlands
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Authors: Katelyn E Mason Brian P Tripet David Parrott Andreas M Fischer Valérie Copié
Publish Date: 2013/02/17
Volume: 8, Issue: 1, Pages: 149-153
Abstract
Leaf senescence is an important process in the developmental life of all plant species Senescence efficiency influences important agricultural traits such as grain protein content and plant growth which are often limited by nitrogen use Little is known about the molecular mechanisms regulating this highly orchestrated process To enhance our understanding of leaf senescence and its regulation we have undertaken the structural and functional characterization of previously unknown proteins that are involved in the control of senescence in barley Hordeum vulgare L Previous microarray analysis highlighted several barley genes whose transcripts are differentially expressed during senescence including a specific gene which is greater than 40fold upregulated in the flag leaves of early as compared to latesenescing nearisogenic barley lines at 14 and 21 days past flowering anthesis From inspection of its amino acid sequence this gene is predicted to encode a glycinerich RNAbinding protein herein referred to as HvGRRBP1 HvGRRBP1 has been expressed as a recombinant protein in Escherichia coli and preliminary NMR data analysis has revealed that its glycinerich Cterminal region residues 93–162 is structurally disordered whereas its Nterminal region residues 1–92 forms a wellfolded domain Herein we report the complete 1H 13C and 15N resonance assignments of backbone and sidechain atoms and the secondary structural topology of the Nterminal RNA recognition motif RRM domain of HvGRRBP1 as a first step to unraveling its structural and functional role in the regulation of barley leaf senescence
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