Journal Title
Title of Journal: World J Microbiol Biotechnol
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Abbravation: World Journal of Microbiology & Biotechnology
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Publisher
Springer Netherlands
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Authors: N S Mendoza M Arai T Kawaguchi T Yoshida L M Joson
Publish Date: 2014/08/01
Volume: 10, Issue: 5, Pages: 551-555
Abstract
Extracellular mannanase from Bacillus subtilis NM39 an isolate from Philippine soil was purified about 240fold with a yield of 73 by ammonium sulphate fractionation DEAEToyopearl chromatography and Sephacryl S200 gel filtration Its M r was 38 kDa and it had a pI of 48 and optimum activity at pH 50 and 55°C It was stable at pH 4 to 9 and below 55°C The amino acid composition of the enzyme was in the order GlyGlxSer and AsxAlaNS Mendoza and LM Joson are with Industrial Technology Development Institute Department of Science and Technology Manila Philippines M Arai and T Kawaguchi are with Department of Agricultural Chemistry College of Agriculture University of Osaka Prefecture Sakai Osaka 593 Japan T Yoshida is with Faculty of Engineering Osaka University Suita Osaka 565 Japan
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