Phylogeny and recombination analysis of Brazilian

Authors: Leilane K Rodrigues Leonardo A Silva Renata M Garcêz Alexandre L R Chaves Ligia M L Duarte José S Giampani Addolorata Colariccio Ricardo Harakava Marcelo Eiras

Publish Date: 2014/08/19

Volume: 44, Issue: 1, Pages: 31-41

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Abstract

The Cowpea aphidborne mosaic virus CABMV causes the most important disease in passion fruit Passiflora spp in Brazil The genetic diversity of Brazilian CABMV isolates infecting yellow passion fruit was evaluated Leaves presenting symptoms of mosaic and blister were collected in orchards in four Brazilian states Samples were submitted to RNA extraction RTPCR sequencing and phylogenetic analyses of two regions of the CABMV genome complete coat protein CP and partial cytoplasmic cylindrical inclusion CI representing together about 20  of the genome Potential alternative hosts to CABMV in areas with intensive passion fruit farming in the state of São Paulo were analyzed Isolates did not infect cowpea Vigna unguiculata and common bean Phaseolus vulgaris Only Macroptilium atropurpureum behaved as a differential host to an isolate from the southernmost region of the state of São Paulo In spite of the little variation in biological properties CABMV isolates from São Paulo presented moderate genetic diversity between one another especially between isolates from the other states with nucleotide identity between 88 and 99  84 to 100  amino acid identity for CP and 85  and 99  93 to 100  amino acid identity for CI Isolates did not group consistently by origin or hosts in the maximum likelihood tree The parent strains of CABMV isolates in São Paulo occupied a more basal position in the phylogenetic tree suggesting the possible origin of Brazilian isolates Recombination events were observed only for CP Isolates from São Paulo were the major parents of CABMV recombinant strains of cowpea passion fruit and peanut from other Brazilian states These results agree with the expansion pattern of passion fruit cultures in the country and suggest that the São Paulo region is the origin of CABMV in Brazil from which isolates spread to other regions and evolvedThis work was supported by a scientific research project given by FAPESP proc 2011/117965 ME is supported by a CNPq research fellowship proc 302018/20115 LKR and RMG were funded by CAPES scholarships LAS was funded by FAPESP scholarship proc 2010/026792 We thank Dr JAM Rezende ESALQ Universidade de São Paulo Brazil and Dr FM Zerbini Universidade Federal de Viçosa Brazil for kindly providing antiserum against CABMV Dr M Moura Rocha Embrapa MeioNorte Brazil for cowpea seeds and Mr Félix Nonnenmacher for the English version and critical review of the manuscript

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