Lineage Tracing in the Mammary Gland Using Cre/lox

Authors: Renée van Amerongen

Publish Date: 2015

Volume: , Issue: , Pages: 187-211

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Abstract

Lineage tracing using Cre/lox technology has become a wellestablished technique to study the contribution of different stem cell populations to organ development and function When used in the mammary gland it forms a valuable addition to the already existing experimental toolbox and an important alternative to other readouts measuring stem cell potential such as the fat pad transplantation assayHere I describe how to set up and analyze an in vivo lineage tracing experiment using tamoxifeninducible Cre/lox technology highlighting the specific challenges that the investigator faces when employing this method and interpreting the results in the mammary glandRvA is supported by the Dutch Cancer Society KWF cancer research career award 20136057 and by a MacGillavry fellowship from the University of Amsterdam I thank my excolleagues and Roel Nusse at Stanford University USA for discussions and feedback during the experimental stages of this work Lauran Oomen and Lenny Brocks at the Netherlands Cancer Institute in Amsterdam the Netherlands for help with the Leica SP5 Ronald Breedijk and Erik Manders for help with analyzing samples on the Nikon A1 JiYing Song for the image depicted in Fig 1b and Amber Zeeman for comments on the manuscript and the image depicted in Fig 1a

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