Localizing Proteins by Tissue Printing

Authors: Rafael F PontLezica

Publish Date: 2015

Volume: , Issue: , Pages: 93-104

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Abstract

The simple technique of making tissue prints on appropriate substrate material has made possible the easy localization of proteins nucleic acids carbohydrates and small molecules in a tissuespecific mode Plant tissues can be used to produce prints revealing a remarkable amount of anatomical detail even without staining which might be used to record developmental changes over time In this chapter we will focus on the protocols for the localization of proteins and glycans using antibodies or lectins probably the most frequently used application but the localization of other molecules is reported and the sources indicated

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