Paper Search Console

Home Search Page Alphabetical List About Contact

Journal Title

Title of Journal:

Search In Journal Title:

Abbravation:

Search In Journal Abbravation:

Publisher

Humana Press, New York, NY

Search In Publisher:

DOI

10.1002/wea.743

Search In DOI:

ISSN

Search In ISSN:
Search In Title Of Papers:

Lineage Tracing in the Mammary Gland Using Cre/lox Technology and Fluorescent Reporter Alleles

Authors: Renée van Amerongen,

Publish Date: 2015
Volume: , Issue:, Pages: 187-211
PDF Link

Abstract

Lineage tracing using Cre/lox technology has become a well-established technique to study the contribution of different (stem) cell populations to organ development and function. When used in the mammary gland, it forms a valuable addition to the already existing experimental toolbox and an important alternative to other readouts measuring stem cell potential, such as the fat pad transplantation assay.Here I describe how to set up and analyze an in vivo lineage tracing experiment using tamoxifen-inducible Cre/lox technology, highlighting the specific challenges that the investigator faces when employing this method and interpreting the results in the mammary gland.RvA is supported by the Dutch Cancer Society (KWF cancer research career award, 2013-6057) and by a MacGillavry fellowship from the University of Amsterdam. I thank my ex-colleagues and Roel Nusse at Stanford University (USA) for discussions and feedback during the experimental stages of this work, Lauran Oomen and Lenny Brocks at the Netherlands Cancer Institute in Amsterdam (the Netherlands) for help with the Leica SP5, Ronald Breedijk, and Erik Manders for help with analyzing samples on the Nikon A1, Ji-Ying Song for the image depicted in Fig. 1b and Amber Zeeman for comments on the manuscript and the image depicted in Fig. 1a.


Keywords:

References


.
Search In Abstract Of Papers:
Other Papers In This Journal:


Search Result:



Help video to use 'Paper Search Console'