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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer Vienna

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DOI

10.1007/bf02692650

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1436-5073

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Impedimetric aptamer-based determination of the mold toxin fumonisin B1

Authors: Xiujuan Chen, Yukun Huang, Xiaoyuan Ma, Fei Jia, Xiaofei Guo, Zhouping Wang,

Publish Date: 2015/05/01
Volume: 182, Issue:9-10, Pages: 1709-1714
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Abstract

We are presenting an aptasensor for the sensitive determination of fumonisin B1 (FB-1) via electrochemical impedance spectroscopy (EIS) and applying aptamer-based biorecognition. A thiolated aptamer for FB-1 was anchored onto the surface of gold nanoparticles (AuNPs) on a glassy carbon electrode. A significant increase in resistance (Ret) is found on interaction with FB-1 in the 0.1 nM to 100 μM concentration range, and the detection limit is as low as 2 pM. The assay was applied to determine FB-1 in spiked maize samples and gave recovery rates ranging from 91 to 105 %. The results demonstrate this method to present new possibilities in the application of aptamers in food safety analysis.The electrodeposited AuNPs create a nanomaterial platform in situ for anchoring of FB-1 aptamers serving as recognition elements. The change in EIS signals induced by aptamer-target interactions was measured and a significant increase in resistance (Ret) is found on interaction with FB-1 in the 0.1 nM to 100 μM concentration range.


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  2. A fluorescent chemical sensor for ethanol determination in alcoholic beverages
  3. Non-enzymatic sensing of glucose and hydrogen peroxide using a glassy carbon electrode modified with a nanocomposite consisting of nanoporous copper
  4. Dynamic evaluation of cell-secreted interferon gamma in response to drug stimulation via a sensitive electro-chemiluminescence immunosensor based on a glassy carbon electrode modified with graphene oxide
  5. Chemometrics to evaluate the quality of water sources for human consumption
  6. Colorimetric and bare-eye detection of alkaline earth metal ions based on the aggregation of silver nanoparticles functionalized with thioglycolic acid
  7. A Pre-Concentration Procedure Using Cloud Point Extraction for the Determination of Manganese in Saline Effluents of a Petroleum Refinery by Flame Atomic Absorption Spectrometry
  8. Graphene nanocomposite modified glassy carbon electrode for voltammetric determination of the antipsychotic quetiapine
  9. Direct electrochemistry of myoglobin based on electrodeposition of Pd nanoparticles with carbon ionic liquid electrode as basic electrode
  10. Direct electrochemistry of myoglobin based on electrodeposition of Pd nanoparticles with carbon ionic liquid electrode as basic electrode
  11. A chemiluminescence assay for L-histidine based on controlled DNAzyme catalytic reactions on magnetic microparticles
  12. Formaldehyde-sensitive conductometric sensors based on commercial and recombinant formaldehyde dehydrogenase
  13. Miniaturized module with biosensors for potentiometric determination of urea
  14. Ionic liquid-based headspace single-drop microextraction coupled to gas chromatography for the determination of chlorobenzene derivatives
  15. New polyacrylate-based lead(II) ion-selective electrodes
  16. Monolithic solid-phase extraction for the rapid on-line monitoring of microcystins in surface waters
  17. Impedimetric aptamer-based determination of the mold toxin fumonisin B1
  18. A Pre-Concentration Procedure Using Cloud Point Extraction for the Determination of Uranium in Natural Water

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