Evaluation of reference genes for quantitative rea

Authors: Qian Ma Zhimeng Zhuang Wenrong Feng Shufang Liu Qisheng Tang

Publish Date: 2015/08/26

Volume: 34, Issue: 10, Pages: 90-97

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Abstract

Differential expression of genes is crucial to growth and development of fish To select the appropriate genes for gene normalization during Cynoglossus semilaevis early developmental process eight candidate reference genes ACTB B2M EF1A GADPH RPL7 TUBA UBCE and 18S were tested for their adequacy by using quantitative realtime PCR The results showed that the expression of all the examined genes exhibited tissue dependent variations in the mature C semilaevis EF1A was listed as the most stable reference among the 14 tissues by RefFinder Furthermore the recommended comprehensive ranking of the stability determined by RefFinder showed that 18S was the most stable gene during the early developmental stages from oosphere to 90 days old in this study However when divided the C t value data of the above mentioned early developmental stages into two separate periods embryo and posthatching periods TUBA and 18S represented the most stable references of these two developmental periods respectively Consequently the reference gene should be carefully and accurately chosen even for studies of the same species at various developmental processes The relevant data may help in selecting appropriate reference genes for mRNA expression analysis and is of great value in the studies of fish growth and developmentFoundation item The National Natural Science Foundation of China under contract No 31201981 China Postdoctoral Science Foundation under contract No 2013M531658 the Special Scientific Research Funds for Central Nonprofit Institutes Yellow Sea Fisheries Research Institutes under contract No 20603022012032

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