Formaldehyde induces the bone marrow toxicity in m

Authors: Guangyan Yu Xiangfu Song Shuhua Zhao Ying Liu Zhiwei Sun

Publish Date: 2015/02/12

Volume: 35, Issue: 1, Pages: 82-86

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Abstract

Formaldehyde FA is a ubiquitous toxic organic compound and it has been regarded as a leukemogen However the mechanisms by which FA induces bone marrow toxicity remain unclear The present study was aimed to examine the bone marrow toxicity caused by FA and the mechanism involving the expression changes of peroxiredoxin3 Prx3 in this process The mice were divided into four groups with 6 mice per group Animals in the control group were exposed to ambient air and those in the FA groups to different concentrations of FA 20 40 80 mg/m3 for 15 days in the separate inhalation chambers 2 h a day At the end of the 15day experimental period all mice were killed Bone marrow cells were obtained The level of hydrogen peroxide H2O2 the apoptosis rate and the activities and protein expression levels of caspase3 and caspase9 were determined by biochemical assay flow cytometry and immunohistochemistry respectively DNA damage and Prx3 expression levels were measured by single cell gel eletrophoresis immunohistochemistry and Western blotting respectively The results showed that the H2O2 level and cell apoptosis rate were significantly increased in FA groups relative to the control group Caspase3 and caspase9 activities and their protein expression levels were markedly increased as well Additionally FA also increased the rate of DNA damage and the expression level of Prx3 compared with control group Our study suggested that a certain concentration of FA causes the bone marrow toxicity by regulating the expression of Prx3

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