The Impact of Hypergravity and Vibration on Gene a

Authors: Markus Wehland Elisabeth Warnke Timo Frett Ruth Hemmersbach Jens Hauslage Xiao Ma Ganna Aleshcheva Jessica Pietsch Johann Bauer Daniela Grimm

Publish Date: 2016/04/14

Volume: 28, Issue: 3, Pages: 261-274

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Abstract

Experiments in space either on orbital missions onboard the ISS or in suborbital missions using sounding rockets like TEXUS as well as parabolic flight campaigns are still the gold standard to achieve real microgravity conditions in the field of gravitational biology and medicine However during launch and in flight hypergravity and vibrations occur which might interfere with the effects of microgravity It is therefore important to know these effects and discriminate them from the microgravity effects This can be achieved by groundbased facilities like centrifuges or vibration platforms Recently we have conducted several experiments with different thyroid cancer cell lines This study as part of the ESACORAGBF 2010203 project focused on the influence of vibration and hypergravity on benign human thyroid follicular epithelial cells Nthyori 31 cell line Gene and in part protein expression regulation under both conditions were analyzed for VCAN ITGA10 ITGB1 OPN ADAM19 ANXA1 TNFA ABL2 ACTB PFN2 TLN1 EZR RDX MSN CTGF PRKCA and PRKAA1 using quantitative realtime PCR and Western Blot We found that hypergravity and vibration affected genes and proteins involved in the extracellular matrix the cytoskeleton apoptosis cell growth and signaling Vibration always led to a downregulation whereas hypergravity resulted in a more heterogeneous expression pattern Overall we conclude that both conditions can influence gene regulation and production of various genes and proteins As a consequence it is important to perform control experiments on hypergravity and vibration facilities in parallel to flight experiments

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