Targeted Metabolic and Genomic Profiling Reveals P

Authors: M Leelambika S Mahesh M Jaheer P K Tripathi P Ranjith Kumar N Sathyanarayana

Publish Date: 2016/06/08

Volume: 9, Issue: 4, Pages: 239-251

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Abstract

Integrating targeted metabolic profiling with genetic diversity estimates is vital for selecting genetic stocks for breeding as well as mapping a biochemical trait In Mucuna pruriens L DC copious amount of LDopa in seeds is viewed both as a boon and bane Our objective of this work was to assess in conjunction both LDopa and genomic diversity in a selected set of germplasm to elucidate their relationship and identify parents for LDopa breeding/mapping The findings revealed good genetic as well as metabolic LDopa diversity among the studied accessions However we could not establish direct relationship between these two as the marker data measured using AFLP and ISSR were not correlated with the seed LDopa contents Based on the consensus information from both the data sets seven parental combinations have been suggested These findings are expected to pave way for genetic improvement as well as genetic mapping of LDopa trait besides integration of molecular markers in M pruriens breeding programsAuthors thank Department of Biotechnology DBT Govt of India for the financial support Sikkim University and Sri Krishnadevaraya Educational Trust Sri KET Bangalore India for the facilities Dr S A Ranade National Botanical Research Institute NBRI Lucknow India and Dr G Ravikanth Ashoka Trust for Research in Ecology and the Environment ATREE Bangalore India for ISSR markers

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